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Digestion by PNGase F following FL-gB denaturation brought on an elecrophoretic change in all of the 3 protein bands, releasing the one hundred and fifty kDa polypeptide backbones of, respectively, the uncleaved precursor and the SU/TM chains, with the two chains co-migrating as a one band. Quantitative deglycosylation by PNGase F also showed that recombinant FL-gB was primarily totally free of c
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The final results shown that IKVpeak was far more sensitive to four-AP (five mM) and IKVsustained was far more sensitive to TEA (five mM) when the membrane prospective was depolarized from 260 mV of keeping potential to +forty mV (Fig. 5A and B). There was no important variation in IKV sensitivity to four-AP among obstruction, regular and sham groups (Fig. 5C P..05). Nevertheless, the IC50 of TEA
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